FastQCFastQC Report
Mon 27 Feb 2023
SRR3128069.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128069.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2124272
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192890.908028727018009No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT60130.2830616794836066No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG38270.18015583691730624No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG30880.14536744823638403No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC27730.13053883871745237No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54800.081.7789461
TACGGGT1150.077.640513
ATAGGGC10400.073.200543
ATAGGGA19300.072.315563
GAATAGG12900.072.177971
AGGGATG27500.071.429275
AGTAAGG11700.070.738521
TAGGGCA8850.070.6221544
AGGGTAC8350.070.348715
CGTAGGG6750.070.315362
TAGGGTC6500.070.127934
ACGGGAT4650.069.731464
GAGGGAT22750.068.991844
AGGGAAT25150.068.947885
AAGAGGG71050.068.918572
ATAGAGG13400.068.782891
AGTAGGG39350.068.429372
ATGAGGG30250.068.353372
TAAGGGA17700.068.232683
TAGGGTA6900.068.105714
Produced by FastQC (version 0.11.5)