FastQCFastQC Report
Mon 27 Feb 2023
SRR3128068.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128068.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632326
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277251.0532510031052384No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT76010.28875602793878874No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG37450.14226961250240283No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG32070.12183141449805229No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29820.1132838409832217No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC26960.10241892531548144No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76750.082.006811
TAGGGTA9250.073.6789864
ACGGGAT6700.073.660024
TAGGGCA12650.073.196964
GAATAGG15800.071.75171
ATAAGGG39100.070.683482
CGTAGGG6600.070.503172
ATAGGGA24100.070.405653
AGTAGGG49850.070.149582
TAAGGGA25100.069.8477553
AGGGATG33900.068.9060755
AGTACGG3550.068.904441
AGGGTAC11800.068.508945
GTACGGG5700.067.6170652
ATAGAGG17500.067.469571
GAGGGAT29350.067.260264
ATAGGGC13500.067.195613
TAGAGGG45950.066.897452
ACGGGTA2250.066.847444
AAGAGGG87850.066.503842
Produced by FastQC (version 0.11.5)