FastQCFastQC Report
Mon 27 Feb 2023
SRR3128067.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128067.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916712
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90320.9852603653055703No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC40450.44125090540976886No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG33830.3690362949323233No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG30910.3371833247519396No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC28830.3144935377741319No Hit
AGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCT11930.1301390185794448No Hit
AAAGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTT11770.1283936503503827No Hit
CGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCT10240.11170356665997608No Hit
TAGACCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCT10140.11061271151681226No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTG254.9220216E-793.994824
GCGATAC156.9032767E-493.994828
CGGGTAA800.088.120145
CGTTTTT29650.086.037651
AGGGATG24850.084.727735
AGGGTAT5500.083.740835
CGAAGGG7400.083.2344362
CGCTACG7450.082.639743
GGAGCTA78950.082.029689
GACCGAT750.081.462178
TCCGCTA7700.080.6858141
ACGACCA7300.080.475017
CTACGAC7700.079.9566355
GCTACGA7750.079.440784
CGACCAA7350.079.288158
ACGGGTA950.079.153534
AGGGAAT17000.079.066235
GGATGGC19950.078.682377
TACGACC7850.078.42886
GGGGGAG91350.077.8402566
Produced by FastQC (version 0.11.5)