FastQCFastQC Report
Mon 27 Feb 2023
SRR3128066.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128066.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875069
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82250.9399258801305954No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37820.4321944898059467No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG37750.43139455288668666No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG32180.3677424294541345No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC31300.3576860796120077No Hit
AGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCT13590.1553020390392072No Hit
AAAGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTT12590.14387436876406318No Hit
CGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCT11260.12867556729812163No Hit
TAGACCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCT11010.12581864972933562No Hit
GGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCT9180.10490601312582208No Hit
ACTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG9150.10456318301756776No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCGCG9050.10342041599005335No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGATG21900.086.2668465
CGTTTTT27250.085.743411
TACACGG550.085.476781
CGCTACG6750.084.2400743
ACGGGTA900.083.543884
TTACGGG1300.083.142222
GGATGGC17300.082.859167
GGAGCTA85750.082.4328849
CGAAGGG6600.081.170462
AGGGAAT16750.080.805265
TCCGCTA7050.080.687661
GAGGGTA4300.079.779544
GCGTAGG1300.079.5591661
ATGGCAC15900.079.218119
AACGAGG2850.079.17851
GATGGCA16400.079.0955358
TACGACC7150.078.8791056
GCATAGG1550.078.859231
GGGGGAG96350.078.778476
GATGACC11450.078.399748