FastQCFastQC Report
Mon 27 Feb 2023
SRR3128065.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128065.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050013
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118881.1321764587676535No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC50110.4772321866491177No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG34040.324186462453322No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG31140.2965677567801541No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC29480.28075842870516843No Hit
AAAGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTT12110.1153319054145044No Hit
TAGACCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCT12070.1149509577500469No Hit
AGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCT12060.11485572083393253No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10830.10314158015186478No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT39350.086.5582351
CGCTACG8200.085.4070743
TCCGCTA8400.082.8890151
CTACGAC8450.082.876285
GAGGGTA6200.082.6335144
GGAGCTA90050.082.5180369
TACGACC8500.082.388786
AGGGATG31850.082.342775
GGTACGC401.035005E-982.250547
ACGACCA8300.081.542717
TAAGCGG2100.080.648771
GCTACGA8700.080.498624
GGATGGC23150.079.991897
AGGGTAT7200.079.639415
AGGGAAT21000.079.45295
AGTACGG1600.079.388631
CGAAGGG8200.079.101852
GGGCGAT2200.079.0459756
CGGGAAT3450.079.015015
TAGGGCA5850.078.739314
Produced by FastQC (version 0.11.5)