FastQCFastQC Report
Mon 27 Feb 2023
SRR3128064.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128064.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365390
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210580.8902548839726219No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG49480.20918326364785508No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG40640.17181099099937008No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC38090.161030527735384No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37180.1571833820215694No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62700.083.306651
AGGGATG45250.074.780265
ATAGCGG4750.074.300521
TAGGGCA13650.073.336474
CGTAGGG7250.073.277322
ATAGGGC13650.071.614963
AGTAGGG40900.071.2684862
GTAGGGA18400.071.006813
GTACGGG7400.070.521322
GAGGGAT33100.070.141014
CGGGTAT2950.070.097695
GGTAAGG13700.070.070281
TAGGGTC6050.069.136554
ACGGGTA3200.069.0273064
CGAAGGG22600.068.857092
ATAGGGA26350.068.846163
TAGGGCG4450.068.6477664
GGATGGC39300.068.164057
AAGGGAT45350.067.982894
AAGGGTA19850.067.9507754
Produced by FastQC (version 0.11.5)