FastQCFastQC Report
Mon 27 Feb 2023
SRR3128063.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128063.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2204252
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188710.856118084502135No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG53010.24048974436679652No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG44250.20074837178326255No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC42520.19289990436665136No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33030.14984675073448953No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT56150.082.685751
CGTAGGG5150.074.829992
AATGCGG4800.074.47931
AGGGATG38250.073.7189255
ACGGGTA2750.071.775434
ATAGGGC15800.071.3876653
GAGGGAT30100.071.0401844
TAGGGTA7750.070.948524
TAGGGCA11900.070.6911854
AGTAGGG38100.070.680342
AGGGAAT26400.070.3157045
ATAGGGA21500.069.9488453
AAGGGTA18150.069.393274
GAATAGG15200.069.3214651
TATAGGG18400.069.218232
CGAAGGG20250.068.696682
GGATGGC33150.068.6137547
ATGCGGG11050.068.475092
AGGGTAC11050.068.473535
ATAGCGG3950.067.8798751
Produced by FastQC (version 0.11.5)