FastQCFastQC Report
Mon 27 Feb 2023
SRR3128062.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128062.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719617
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275551.01319413726271No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG51940.1909827744127206No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG46090.16947239261999023No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC44480.16355244139156358No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC44300.1628905834902488No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77700.083.977691
AGGGATG53950.075.705855
AGTAGGG49350.073.335232
AGGGTAC13900.073.036525
GGATGGC45800.072.758187
ACGGGTA3750.072.693874
GAATAGG17900.070.435291
ATAGGGA32400.069.4850853
AAGGGAT47900.069.470254
AGGGAAT35650.069.346935
ATAGGGC18100.069.332013
TAGGGTA12400.068.984384
GAGGGAT41850.068.8444
AGGGCAT24150.068.70035
TAGGGCA15400.068.364144
CGTAGGG8050.068.312322
TAAGGGA24700.068.312253
ACGGGAT10600.068.283524
AAGGGTA23300.068.180764
ATAGCGG4850.067.8992161
Produced by FastQC (version 0.11.5)