FastQCFastQC Report
Mon 27 Feb 2023
SRR3128061.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128061.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2822796
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT358841.271221866546502No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43270.15328773315535377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT108550.085.5620351
AGGGATG53400.071.815815
AGTAGGG47400.071.621842
GTACGGG5450.069.8835752
GAGGGAT38950.069.2588964
AAGGGAT51800.069.044024
AGAGGGC39700.069.015913
AAGGGTA22900.067.72514
ATAGGGA29950.067.474983
TAAGGGA25750.067.164813
GAATAGG19500.067.077051
TAGCGGG14450.067.032592
ATAGGGC20650.066.6835563
GATAGGG42100.066.677432
AAGAGGG95800.066.211452
TAGGGAT23900.065.874624
TAGAGGG52950.065.535562
AGGGTAC16150.065.475855
AGGGTAT16450.065.138865
TATAGGG23650.065.013462
Produced by FastQC (version 0.11.5)