FastQCFastQC Report
Mon 27 Feb 2023
SRR3128060.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128060.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2614104
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324681.2420316865740613No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38940.14896117369469616No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG30940.11835795362388031No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC29740.11376747061325793No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG29680.1135379464627268No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT100600.086.627311
AGGGTAC14450.072.8506555
GAGGGAT36400.072.5571064
AGGGATG48500.072.382265
TAAGGGA26350.070.803523
TAGACGG3550.070.2141951
ATAGGGA24300.070.201333
AAGGGAT44200.068.896594
AGTAGGG43200.068.859672
ATAGCGG5950.068.766941
GATAGGG36350.068.26132
ATAGGGC17500.068.208843
AGAGGGC38200.067.53893
AAGGGTA18500.067.316134
AGGGCAT22100.067.1967245
AGATAGG14850.067.140851
AAGAGGG87600.067.00442
AGTTAGG8250.066.6974641
TAGGGAT19200.066.3304754
TAGAGGG49600.066.227832
Produced by FastQC (version 0.11.5)