FastQCFastQC Report
Mon 27 Feb 2023
SRR3128059.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128059.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3282807
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT471711.4369105463708345No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC50500.1538317665339449No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT140300.085.510781
AGGGTAC16800.072.458945
AGGGATG62900.072.181855
ATAGGGA32800.069.786253
GAATAGG19300.069.718341
ATAGGGC21200.069.394213
AGTAGGG56850.069.0352252
AAGGGAT62250.068.331974
CGTAGGG8100.067.891532
TAGAGGG59800.067.75182
TAAGGGA33200.067.388173
AAGGGTA25200.067.3319854
GAGGGAT46150.066.7089844
TAGGGTA12000.066.585924
GATAGGG51750.066.392922
TAGGGCA20350.066.287594
GTAGGGA25550.066.225673
AAGAGGG113200.066.059982
AGGGCAT27550.065.851735
TAGGGAT27300.065.768134
Produced by FastQC (version 0.11.5)