FastQCFastQC Report
Mon 27 Feb 2023
SRR3128055.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128055.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2646290
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322761.2196698018735666No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG31110.11756081155126612No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG30210.11415982375325455No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29130.1100786383956407No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC27190.10274762025326023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94500.084.65471
CGTAGGG8400.077.246822
TAAGGGA23600.073.682663
AGGGATG43100.072.731775
AGTAGGG42150.072.175122
TAGGGCA14000.071.167784
AGTAAGG18800.070.336781
AGGGAAT30150.069.678025
GAGGGAT37750.068.473314
ATAGGGC16250.067.965753
AAGGGTA18600.067.716964
GTAGGGA19600.067.618993
AGGGTAC13000.067.6042255
GTAGGGT11500.067.431333
TAGAGGG45850.067.2737052
AGAGGGC37800.067.139423
AGGGCAT20800.066.881195
AAGAGGG91450.066.78922
ACGGGAT9150.066.772534
GGTAAGG17000.066.711711
Produced by FastQC (version 0.11.5)