FastQCFastQC Report
Mon 27 Feb 2023
SRR3128054.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128054.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2496657
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293121.1740499395792052No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG36460.14603527837424204No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG32610.13061465792057136No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC28560.11439296627450227No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25910.10377877297522246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86800.084.587041
AGGGATG35850.073.806035
TAGACGG3500.072.56821
CGTAGGG6550.072.469062
TAGGGTA6950.071.679284
AGTAGGG41100.071.353522
AGTAAGG16350.071.0558551
TAAGGGA23750.070.842193
AGGGTAC11150.069.969035
AAGGGTA18400.069.729644
ATAGCGG4650.068.782291
GAGGGAT29150.068.3596654
ATGCGGG13450.067.787882
AGGGAAT29800.067.1840065
AGATAGG13800.067.144091
TAGGGCA11550.067.1389544
GTAGGGA17300.067.100143
GGTAAGG15600.066.934341
GACCGAT2200.066.222098
GTAGGGT9600.066.0899053
Produced by FastQC (version 0.11.5)