FastQCFastQC Report
Mon 27 Feb 2023
SRR3128053.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128053.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3107140
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417421.3434219249856783No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG34370.11061619367006313No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG34340.11051964185714194No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34190.11003688279253589No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT117150.084.054371
CGTAGGG10400.075.019562
AGGGATG48650.074.5796365
TAAGGGA30700.074.2511443
AGTAGGG50250.072.394352
GGTAAGG20100.071.624021
GTAGGGA24950.070.264843
TAGGGTA10200.070.039534
TAGGGCA15550.069.517994
GTAGGGT12900.068.860773
AGGGAAT36200.068.680415
ATAGGGC18750.068.4323043
GAGGGAT39650.068.396274
AGTAAGG20300.068.369011
ACGGGTA4400.068.363944
ATAAGGG42600.068.293732
TAGAGGG53350.068.1877442
ATAGGGA30650.068.085143
AAGGGTA23150.068.013264
AAGAGGG107800.066.4455642
Produced by FastQC (version 0.11.5)