FastQCFastQC Report
Mon 27 Feb 2023
SRR3128049.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128049.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2576689
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231080.8968098206652024No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC42530.16505678411325542No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34940.13560037707305772No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG31670.12290967206364446Illumina PCR Primer Index 8 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74650.082.840621
AGGGTAC13350.078.8564765
CGTAGGG6750.077.3155442
TAGGGTA9500.073.216454
ATAGGGA26350.069.915923
ATAGGGC16350.069.848833
TAAGGGA22350.069.391623
AAGGGTA21800.069.202084
AGGGATG35300.068.964515
AGTAGGG39350.068.702212
GAGGGTA17850.068.4550864
AGGGTAA14600.067.9203955
AGATAGG14500.067.835351
AGGGCAT19300.067.451725
GATAGGG39900.066.4592
AGTAAGG15400.066.315771
TAGGGCG3550.066.193034
TAGAGGG45350.066.144092
TAGGGCA14950.066.015924
ATAGGGT15400.065.3075943
Produced by FastQC (version 0.11.5)