FastQCFastQC Report
Mon 27 Feb 2023
SRR3128048.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128048.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2427833
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213290.8785200629532591No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39440.16244939417167492No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG35600.14663282029694794Illumina PCR Primer Index 8 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32490.1338230430181977No Hit
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG26120.10758565354371573Illumina PCR Primer Index 8 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC25080.10330199811931051Illumina PCR Primer Index 8 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66650.084.257481
CGTAGGG7000.075.863992
TAGGGTA10050.074.818544
AGGGTAC14750.074.238335
TAGGGCG4200.073.849914
AGGGATG36500.072.232775
ATAGGGA23900.071.181313
TAAGGGA22350.070.2302553
ATAGGGC14500.069.358723
TAGGGAT15650.069.066724
AGTAGGG40250.068.7709052
AAGGGTA19550.068.7503054
TAGCGGG12450.067.945152
AGGGAAT29150.067.874695
GTAGGGA20100.067.5704963
AGGGTAA13300.066.784315
GTAGGGT11050.066.771743
TAGGGCA11900.066.741364
AGGGACT30150.066.55875
AAGAGGG78950.066.430482
Produced by FastQC (version 0.11.5)