FastQCFastQC Report
Mon 27 Feb 2023
SRR3128047.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128047.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3023180
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303411.0036120905801176No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC51750.171177369524805No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40060.1325094767761099No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG32860.10869349492918053Illumina PCR Primer Index 8 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92300.083.53821
CGTAGGG9200.076.6292342
AGGGTAC15300.076.1818165
TAAGGGA28550.071.939333
AGGGATG45000.071.021125
ATAGGGC17050.070.292153
TAGGGCG5550.070.2871864
GAATAGG19750.070.030651
TAGGGTA10900.068.9897464
GTAGGGA24700.068.691233
ATAGGGA32500.068.5466163
AGTAGGG49600.067.750962
AAGGGTA24600.067.633094
CTACGAC11600.067.257575
ATAGCGG7150.067.112281
AATAGGG48250.066.919162
TAAGAGG22400.066.786231
TAGGGAT21350.066.48144
GAGGGTA21050.066.31254
AGGGTAA18650.066.025775
Produced by FastQC (version 0.11.5)