FastQCFastQC Report
Mon 27 Feb 2023
SRR3128045.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128045.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121620
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108090.9636953691981242No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG60970.543588737718657Illumina PCR Primer Index 5 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG51340.4577307822613719Illumina PCR Primer Index 5 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC51230.456750057951891Illumina PCR Primer Index 5 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31630.2820028173534709No Hit
AGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT21220.189190634974412Illumina PCR Primer Index 5 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT20280.18080989996612043No Hit
CGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT17280.15406287334391325Illumina PCR Primer Index 5 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT17140.15281467876821025Illumina PCR Primer Index 5 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG15060.13427007364347998Illumina PCR Primer Index 5 (95% over 22bp)
AGAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT13770.1227688521959309No Hit
ACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT13600.12125318735400582Illumina PCR Primer Index 5 (95% over 21bp)
GAAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT12740.11358570638897308No Hit
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG11940.10645316595638453Illumina PCR Primer Index 5 (95% over 22bp)
GGAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT11600.10342183627253436No Hit
GGAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT11440.10199532818601666No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG2400.088.115892
CGTTTTT32750.085.146631
CGGGTAA800.082.245165
AGGGACG1500.081.4618765
AGGGATG25050.081.4243555
AAGGGCA13800.079.346874
AGGGAAT23350.079.3015445
AAGGGTA9600.078.8147664
TACGGGT850.077.403763
TAGACGG1400.077.254521
GGAGCTA33200.077.149079
TAAGGGA11450.076.751893
GTACGGG2150.076.5037162
GGTAAGG6650.076.37061
CGAAGGG9700.075.579812
CGCTACG5850.075.5135653
TAGGGCG1000.075.192224
AAGGGCG3900.074.710234
GGGAATG27650.074.277736
GCGGGAT4050.074.263924
Produced by FastQC (version 0.11.5)