FastQCFastQC Report
Mon 27 Feb 2023
SRR3128044.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128044.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1351673
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161071.191634367187922No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG58530.43301893283360693Illumina PCR Primer Index 5 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG53940.3990610154970914Illumina PCR Primer Index 5 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC51440.3805654178192507Illumina PCR Primer Index 5 (95% over 23bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC40400.29888885847390606No Hit
AGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT21580.1596539991551211Illumina PCR Primer Index 5 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT20560.15210779530256208No Hit
CGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT17330.12821148310279187Illumina PCR Primer Index 5 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG15430.11415482886763292Illumina PCR Primer Index 5 (95% over 22bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15320.11334102256980794No Hit
GGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT15080.11156544519273523Illumina PCR Primer Index 5 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT15020.11112155084846705Illumina PCR Primer Index 5 (95% over 21bp)
AGAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT13710.10142985766527851No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC13710.10142985766527851No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT48350.085.808581
TACGGGT1250.082.721373
CGTAGGG3700.080.028342
AGGGATG28650.079.890165
AGGGAAT30300.079.107295
ACGGGTA2250.077.290164
AGACGGG7750.077.020632
TAAGGGA15100.076.8820653
CTAGCGG1050.076.158481
AAGGGTA13150.075.773124
CGAAGGG9750.075.6833042
GGAGCTA34200.075.3083959
TAGGGCG2250.075.201244
TAGCGGG5150.074.836192
CGGGAAT3400.074.645535
GGATGGC23500.074.398487
TAGGGCA7600.074.2117544
CGCTACG7800.074.116613
AAGGGCA16350.073.878894
AAGCGGG13250.073.782362
Produced by FastQC (version 0.11.5)