FastQCFastQC Report
Mon 27 Feb 2023
SRR3128043.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128043.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2876933
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242650.8434329197099829No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC55770.1938522725416268No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG45470.15805025699242908No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC36530.12697549786526138No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG34630.1203712425697783No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31570.10973491562021084No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT82700.081.03391
ACGGGTA3800.074.208884
AGGGCAT28600.072.1417545
AGGGATG55900.070.7086265
AGTAGGG48900.070.00512
TAGGGCA16300.069.7775964
GTAGGGT14100.069.6651153
ACGGGAT13000.069.413844
TAGGGTC9650.069.159084
GAGGGAT43550.069.0685044
GGTAAGG16650.068.9664461
CGGGTAT3650.068.2450565
GAATAGG17600.068.185141
ATAGGGA32650.068.087313
GGATGGC44150.067.5975957
CGTAGGG9200.067.467292
TAGGGTA15450.067.228284
AGGGAAT36850.067.086715
AGAGGGC40600.067.0256043
AGGGTAC16450.066.855665
Produced by FastQC (version 0.11.5)