FastQCFastQC Report
Mon 27 Feb 2023
SRR3128042.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128042.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2648199
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220830.8338874835312603No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG49540.1870705335966066No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC48990.18499365040165033No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC40910.15448234819211093No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG37910.14315389440144038No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28430.10735598042292138No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65500.083.856841
TAGGGCG4700.072.993324
CGTAGGG8550.072.5547562
AGGGATG50300.072.0365145
AGTAGGG42750.071.125652
GGTAAGG15300.070.999861
GAGGGAT39150.070.463534
TAGGGCA12100.070.299354
ATAGGGA29150.070.13083
TAGGGTC8800.069.9594964
GAATAGG15900.069.799421
AGGGCAT23150.069.023065
AATGCGG5050.068.909211
GGATGGC39150.068.665537
TAAGGGA24000.068.5353853
AAGGGAT54100.068.104674
TAGGGTA12200.067.411864
AGGGAAT35150.067.3862845
AGGGTAA15450.067.2248845
ATGGCAC35100.067.215759
Produced by FastQC (version 0.11.5)