FastQCFastQC Report
Mon 27 Feb 2023
SRR3128041.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128041.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3314120
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317240.9572375170482662No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC63600.1919061470314895No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG49000.14785222019721678No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC44090.13303682425500585No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG40640.12262682099622223No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36450.10998394747323574No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96000.082.75881
CGTAGGG10950.072.96762
AGGGATG66600.071.275855
GAGGGAT50350.071.1285864
TAGGGCA17100.070.910784
TAAGGGA33750.070.046983
AGTAGGG54000.069.890312
ACGGGAT12350.069.642254
GGTAAGG19350.069.038771
AGGGTAA21400.068.742575
GAATAGG20550.068.44081
ACGGGTA5050.067.9391564
ATAGGGC22000.067.722313
CTACGAC12500.067.679595
TAGCGGG15900.067.100222
AGGGTAC19500.067.004725
ATAGGGA35450.066.8204653
TAGGGTC11750.066.7985844
AGGGCAT31250.066.777195
AAGGGTA30450.066.678394
Produced by FastQC (version 0.11.5)