FastQCFastQC Report
Mon 27 Feb 2023
SRR3128040.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128040.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2274453
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227020.9981300998525798No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33590.1476838606909002No Hit
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG26400.11607186431199061RNA PCR Primer, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64250.082.604281
CGTAGGG6450.077.255712
ATAGGGA20700.071.293223
ATAGGGC12700.070.6835863
TAAGGGA20000.070.498553
AGTAGGG40200.069.3446662
GAATAGG15350.069.273421
TATAGGG17850.068.999742
TAGGGCG4100.068.7790764
TGTAGGG17050.068.377262
AGTAAGG15650.068.246131
ATAAGGG32650.067.670452
GTAGGGA18950.067.212343
AAGAGGG81600.067.115092
GGTAAGG12800.066.900281
ATGTAGG7900.066.7048651
TAGAGGG40900.066.663652
ACGGGAT6950.066.272024
TAGTAGG10100.066.1505661
AGGGATG32450.066.044875
Produced by FastQC (version 0.11.5)