FastQCFastQC Report
Mon 27 Feb 2023
SRR3128039.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128039.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2106318
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205060.9735472041733491No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29990.14238115991982217No Hit
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG29720.14109930219463537RNA PCR Primer, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG23550.11180647936351491RNA PCR Primer, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59900.082.756121
CGTAGGG5500.072.6311342
ATAGGGC11450.072.239343
GAATAGG13650.070.977531
TAAGGGA17900.070.888753
GCGATCG200.002163848170.494938
ATAAGGG28900.069.437912
ATAGAGG14200.069.2220151
ATAGCGG4400.068.409061
AGTAGGG36800.068.0684742
ATAGGGA17550.067.750113
GAGGGAT22500.067.675134
AGGGATG28450.066.571665
ACGGGAT5950.066.348174
AGGGAAT21200.066.282975
GATAGGG31500.065.795262
TAAGAGG17050.065.374791
AGGGATA20050.064.927995
GGTAAGG11450.064.898991
TATAGGG16350.064.674242
Produced by FastQC (version 0.11.5)