FastQCFastQC Report
Mon 27 Feb 2023
SRR3128037.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128037.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2897059
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273940.9455796378327124No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC51230.17683450699485237No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG31750.10959390195367094No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31750.10959390195367094No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85950.084.0502241
AGGGATG56050.073.283235
ATAGCGG6000.072.9471051
AGTAGGG46650.072.362592
GTAGGGA24000.071.670213
CGTAGGG8150.071.532822
GGGCGAT10700.071.154126
TAGGGCA15550.070.721964
GAATAGG17900.069.9366761
ATAGGGC16950.069.871443
AGTAAGG19050.068.92641
GAGGGAT37550.068.461474
CTACGAC10950.068.242015
AGATAGG15450.068.233231
GGTAAGG16350.068.219251
TAGGGTA11450.067.724734
AAGGGTA19950.067.1383744
TAAGGGA29400.067.138373
AAGGGAT57750.067.056994
CGAAGGG27850.066.851512
Produced by FastQC (version 0.11.5)