FastQCFastQC Report
Mon 27 Feb 2023
SRR3128036.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128036.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2697978
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254560.9435214075133304No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC44540.165086594479273No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG34110.1264280138681635No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29010.1075249686987811No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG28440.10541227541514422No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80850.083.5877461
GAATAGG16350.076.512051
ATAGGGC15750.076.0829543
AGGGATG48450.074.975875
TAGGGCA14850.074.36514
AGTAGGG42950.072.758922
GTAGGGA24050.071.3190463
ATAGCGG4750.071.286091
AGATAGG14100.071.043841
GTAGGGC13650.070.918953
GTACGGG6300.070.115662
AGTAAGG17900.069.8866961
CGTAGGG7950.069.749742
TAAGGGA27000.069.4443363
ATAGGGA28000.068.978153
TAGAGGG52450.067.46482
AATAGGG44500.067.373392
GAGGGTA16350.066.967784
GGTAAGG15050.066.8718261
AGGGAAT30200.066.599755
Produced by FastQC (version 0.11.5)