FastQCFastQC Report
Mon 27 Feb 2023
SRR3128035.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128035.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3387055
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361321.0667674425127434No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC61170.18059937024937592No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36180.1068184602848197No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT109450.083.336821
CGTAGGG10400.074.114062
ACGGGTA4200.073.855824
ATAGGGC20250.073.10983
AGGGATG60600.072.2878655
AGTAGGG53900.071.239912
TAGGGCA18450.070.817164
GTAGGGA30950.070.460773
GAGGGTA22550.070.446644
TAGGGTA12000.069.323764
TAGAGGG57400.068.697382
ATAGGGA36150.068.646073
AAGGGTA25050.068.106574
GTACGGG6950.067.624692
AGGGAAT39100.067.558765
AGTAAGG21800.067.540271
AGGGTAC18700.067.362055
GAATAGG22500.067.320641
CGAAGGG32450.067.203732
AATAGGG54900.066.860372
Produced by FastQC (version 0.11.5)