FastQCFastQC Report
Mon 27 Feb 2023
SRR3128034.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128034.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2176034
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT773533.5547698243685533No Hit
CGTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT66340.3048665599894119No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCT62240.28602494262497735No Hit
CGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT61660.2833595430953744Illumina PCR Primer Index 3 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG40100.18428020885703073TruSeq Adapter, Index 14 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35170.16162431285540574No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTC29830.13708425511733732No Hit
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC27810.12780131192803054Illumina PCR Primer Index 3 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC26760.12297601967616315No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT225400.089.876431
TAGGGTA7500.075.822924
ACGGGTA2550.075.56494
AGGGATG31850.075.550425
ATAGGGC13650.074.025643
TAGGGCA9250.073.1639564
ATAGGGA19650.070.556313
AGTAGGG35050.070.156792
ATAGCGG4550.069.2771
GAGGGAT25200.068.631534
AGGGACT25250.067.378855
AGGGTAC9850.067.275875
CGTTTCT10700.067.271851
AGAGGGC34900.067.062593
GTAGGGC11950.066.858633
ATAGAGG15500.066.775431
GTAGGGT9150.066.772663
AGATAGG12150.066.213351
ATAGGGT9450.066.144883
TAGAGGG37150.065.305082