FastQCFastQC Report
Mon 27 Feb 2023
SRR3128033.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128033.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052992
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT704703.4325511253818815No Hit
CGTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT70380.34281672797555957No Hit
CGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT65430.31870557703098695Illumina PCR Primer Index 3 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCT65270.31792622669742504No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG44160.2151006920630962TruSeq Adapter, Index 14 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32090.15630845127501714No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTC30630.14919687948126442No Hit
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC30010.1461768969387119Illumina PCR Primer Index 3 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC28280.13775017145707338No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT202350.089.99961
ATAGGGC14400.075.386213
TAGGGCA10450.074.650754
AGGGATG30550.071.838735
AGTAGGG33000.071.345432
AGGGTAC9050.070.622535
ATAGCGG3550.070.201091
AGTAAGG11200.070.112391
TAGGGTA6250.069.927064
CGTAGGG6150.069.5358352
AGAGGGC30850.068.853443
ATAGGGA15600.068.683533
AGGGCAT14800.068.58755
TAGTAGG10600.068.3142241
GTACGGG3100.068.2170942
ATAGAGG13200.068.038661
GAGGGTA11400.068.0176244
GTAGGGT9250.067.569753
GAATAGG12100.067.229061
TATAGGG14450.066.9948962
Produced by FastQC (version 0.11.5)