FastQCFastQC Report
Mon 27 Feb 2023
SRR3128032.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128032.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2500044
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970753.8829316604027766No Hit
CGTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT68990.2759551431894799No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCT67570.27027524315572043No Hit
CGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT60960.24383570849153055Illumina PCR Primer Index 3 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG45220.1808768165680284TruSeq Adapter, Index 14 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40260.16103716574588287No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTC31270.12507779863074409No Hit
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC29380.11751793168440236Illumina PCR Primer Index 3 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC28030.11211802672272968No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT276100.089.982021
AGTAGGG40650.072.495192
ATAGGGC16600.072.199523
AGGGATG37250.071.0397955
TAGGGCA11350.070.811294
TAGGGCG3950.070.203234
GTAGGGT11150.069.130733
TAGGGTC6600.069.076454
ATAGGGA22250.068.863623
AGAGGGC40600.068.416943
TAGGGTA8700.068.069654
ATGTAGG7900.067.2783051
GAGGGAT27800.066.6121754
CGTTTCT11350.066.305241
TAAGGGA21400.066.108143
GAGGGTA14300.066.06364
AAGGGTA15800.065.7411654
GTTTTTT380550.065.56972
AGGGACT31800.065.330315
ATAACGG2450.065.273451