FastQCFastQC Report
Mon 27 Feb 2023
SRR3128031.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128031.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597511
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67111.1231592389094092No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG30620.512459184851827No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG28020.4689453415920376No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC26140.4374814856964977No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC11970.20033103993064563No Hit
AGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT10190.17054079339125136No Hit
CGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT9560.15999705444753318No Hit
GGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT9080.1519637295380336No Hit
AAAGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT8910.14911859363258584No Hit
AGAGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT7630.12769639387392032No Hit
ACCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT7390.12367973141917052No Hit
ACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG7340.12284292674109765No Hit
TCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG6610.11062557844123372No Hit
GAAGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT6000.10041656136874468No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG950.089.088322
CGTTTTT21500.085.361451
TAAGCGG1350.083.659071
ATAGCGG1250.082.822491
GACCGAT401.035005E-982.2485358
CGAAGGG3100.081.903782
TAAGGGA5000.081.778543
CTAACGG700.080.671261
TAGCGGG2850.079.189612
CTACGAC2500.078.9585955
GTAGCGG600.078.43041
AGGGTAC2050.077.949845
TAGGGTA2050.077.949844
AGGGAAT13300.077.742985
GGGTATC2850.077.507396
AGGGATG11500.077.24215
GGGAATG15000.076.76536
AGGGTAT4300.076.510275
GGAGCTA12050.076.446779
AGCGGGA3650.075.9712453
Produced by FastQC (version 0.11.5)