FastQCFastQC Report
Mon 27 Feb 2023
SRR3128030.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128030.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571992
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60751.0620777912977803No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG34730.6071763241443936No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG29900.5227345837004713No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC28300.49476216450579724No Hit
AGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT10810.18898865718401656No Hit
CGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT10300.1800724485657142No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC10250.17919831046588064No Hit
AAAGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT10020.17517727520664625No Hit
GGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT9560.16713520468817747No Hit
ACCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT8490.1484286493517392No Hit
ACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG8450.14772933887187234No Hit
AGAGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT8190.1431838207527378No Hit
TCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG6530.11416243583826347No Hit
AATGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT6470.1131134701184632No Hit
GAATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT6450.11276381487852977No Hit
GGAACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT6380.11154002153876277No Hit
GATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC6260.10944209009916223No Hit
GCCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT6010.1050713995999944No Hit
GAAGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT5940.10384760626022743No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG1500.087.7278754
CGTAGGG900.083.5503542
AGGGAAT14200.083.079555
CGTTTTT20500.082.359791
CGGGTCC401.035005E-982.252075
ACGGGTC401.035005E-982.244874
AATGCGG1100.081.2335741
AGTAAGG2900.081.086141
TCCGCTA2500.080.891531
CGAGGGA3000.079.895033
CGGGAAT1950.079.540465
TATAGGG4550.078.50062
CGAAGGG3300.078.3284452
GGGAATG17050.078.289366
AGCGGGA2950.078.0629353
GTAGGGT1750.077.880863
AGGGATG11850.077.740355
CGGGTAT551.8189894E-1276.9110265
CGGGTAC551.8189894E-1276.9110265
AGTAGGG8150.076.69462
Produced by FastQC (version 0.11.5)