FastQCFastQC Report
Mon 27 Feb 2023
SRR3128029.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128029.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences685446
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86831.2667664557091296No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG32850.4792500065650686No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG30590.44627877323669557No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC27350.399010279438497No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC13900.20278767401079004No Hit
AGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT11060.16135479673088762No Hit
CGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT10770.15712397475512294No Hit
AAAGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT9640.14063835809093642No Hit
GGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT8620.12575753596928133No Hit
AGAGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT8350.12181849481943142No Hit
ACCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT8160.11904657697324077No Hit
ACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG7590.11073082343466881No Hit
GAATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT6950.10139383700539502No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG1500.087.742662
CGAAGGG4450.086.615952
CGGGTAA600.086.163255
CGTTTTT28300.084.955331
ATAGCGG1450.084.3648451
TAGCGGG3400.081.56752
ACGGGTA1450.081.04314
AGGGATG13750.080.66595
AGGGAAT15300.078.330235
ATGTAGG2550.077.493491
AGGGTAC3300.076.9060445
GGATGGC10400.076.823887
GGGTACG800.076.371976
AGTAGGG10150.075.948962
AGGGTAT4600.075.6057055
TAAGGGA6250.075.2083
CGAGGGA3750.075.207993
TTCGCGA255.5588687E-575.197029
CGGGAAT2200.074.769775
AAGGGTA5100.074.6554
Produced by FastQC (version 0.11.5)