FastQCFastQC Report
Mon 27 Feb 2023
SRR3128028.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128028.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2682409
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225020.8388728191711257No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39620.1477030534866234No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG32610.12156982771829351No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32440.12093606903346955No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71550.081.1144261
AGGGATG42900.073.073015
ATAGGGA27300.071.445343
GAATAGG20450.071.122971
ATAGGGC17900.070.3671953
AGGGAAT32450.069.520845
GAGGGAT36050.069.357724
CGTAGGG8800.068.927082
AAGAGGG88100.067.9948962
ATAGCGG5400.067.990081
AGTAGGG45450.067.0384452
AGGGACT42000.067.02955
ACGGGTA2950.066.913814
AAGGGCG10550.066.8232044
CGAAGGG26500.066.7151952
TAGCGGG14000.066.499742
AGAGGGC38850.066.415553
TATAGGG21550.066.3298652
GATAGGG38800.066.2885362
ATAAGGG38950.066.033252
Produced by FastQC (version 0.11.5)