FastQCFastQC Report
Mon 27 Feb 2023
SRR3128027.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128027.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2494576
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205860.8252304199190563No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG35870.14379197106041267No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35190.14106605691708732No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30410.12190448396841788No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG27940.11200300171251548No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGC26900.10783395655213553No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65800.081.630581
ATAGGGA24950.075.534763
AGGGATG38750.074.345235
ATAGGGC15550.072.536053
TAGGGCA12350.071.541044
ATAGCGG4700.071.051481
TAGACGG2200.070.5511251
GAATAGG17250.069.255981
AGTAGGG43400.069.196512
AGATAGG13750.069.097341
TATAGGG19200.069.027312
CGTAGGG8800.068.893792
CGAAGGG21450.067.9215242
TAAGGGA20000.067.911123
ACGGGTA3050.067.798054
GATAGGG34250.067.648682
AGGGAAT29450.067.023695
TAGGGAT20750.066.8141254
GTAGGGA20700.066.5227663
AGGGACT35950.066.278615
Produced by FastQC (version 0.11.5)