FastQCFastQC Report
Mon 27 Feb 2023
SRR3128026.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128026.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3134144
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291710.9307485552674032No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT44710.1426545812828No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38820.12386157113393641No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTG34620.11046078291233588No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92150.082.1341551
AGGGATG52000.072.849655
CGTAGGG10700.072.4808962
ATAGGGA31450.071.438233
ATAGCGG5400.071.430421
GAATAGG20800.069.880861
AGTAGGG50750.069.3696062
TAGGGTC8250.068.937394
TATAGGG26100.068.793322
ATAGGGC19500.068.214283
AGGGACT45600.067.61375
ACGGGTA4000.066.978944
GAGGGAT43700.066.5782854
TAGAGGG58600.066.413472
GTAGGGT13600.066.356853
CGAAGGG30600.066.203252
TAGGGAT24900.065.879384
AGGGAAT38500.065.799685
AAGAGGG108250.065.5650562
GTAGGGA23100.065.519013
Produced by FastQC (version 0.11.5)