FastQCFastQC Report
Mon 27 Feb 2023
SRR3128025.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128025.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425610
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177400.731362420174719No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG38610.1591764545825586No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38050.15686775697659558No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG31700.1306887751946933No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC26280.10834388050840821TruSeq Adapter, Index 27 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24520.10108797374681008No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57650.080.4789351
AGGGATG46050.075.012545
CGTAGGG7950.072.7418442
AGTAGGG42200.070.858342
GAGGGAT36450.070.786584
AGAGGGC32950.070.318183
TAGGGAT22250.070.126834
AGGGCAT20350.069.0530555
TAGAGGG44850.068.8730242
CGAGGGA15600.068.688873
TACGGGT1850.068.591173
ATAGGGC14900.068.446243
ACGGGTA2750.068.360224
GTAGGGA24300.068.272313
AAGGGAT44800.067.9787454
ATAAGGG31900.067.797462
TAGGGTA10950.067.384764
TAAGGGA22150.066.836393
AGGGAAT31850.066.696825
AGGGTAA13600.066.349625
Produced by FastQC (version 0.11.5)