FastQCFastQC Report
Mon 27 Feb 2023
SRR3128024.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128024.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244519
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160780.7163227399723504No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG41760.18605322565770216No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36120.16092534747979412No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG36100.16083624152880863No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC30680.13668852881174096TruSeq Adapter, Index 27 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT49950.079.756851
AGGGATG45250.075.713075
GAGGGAT34700.073.5414664
AGTAGGG37200.073.2735062
AGGGCAT16900.070.077055
GTAGGGA20800.069.3644563
TAGGGCA13350.069.3500754
AGAGGGC32700.068.84143
AAGAGGG72550.067.9516452
TAAGGGA21400.067.639273
AGGGAAT29900.067.586395
ATAAGGG30850.067.4855042
ATAGGGC14350.067.46483
GGTAAGG11900.067.192691
TAGGGAT20650.066.909724
ATAGGGA25050.066.788893
AAGGGAT42800.066.2118154
TAGCGGG10050.065.9348452
ATGAGGG33450.065.6119162
GGATGGC34700.065.550777
Produced by FastQC (version 0.11.5)