FastQCFastQC Report
Mon 27 Feb 2023
SRR3128023.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128023.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2794324
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233200.8345488926838834No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT45930.16436891355476316No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG40600.14529453277429533No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG37180.13305543666375122No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC30470.10904247324218666TruSeq Adapter, Index 27 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28190.10088307583515728No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71300.080.346241
AGGGATG53100.075.675075
AGTAGGG47800.071.578852
TAGGGCA14250.071.569194
GAGGGAT43600.070.389514
ATAGGGC17100.069.810193
AGGGCAT24300.069.4335565
GTAGGGA28100.069.410163
TAGAGGG53250.068.842442
ATAAGGG39200.068.69892
AGGGTAT15600.068.689685
ACTAGGG13900.068.6376652
ACGGGTA3850.067.140294
TAGCGGG13150.066.833952
TAGGGTA10350.066.751074
AGAGGGC40350.066.6244663
TAAGGGA27800.066.608963
AGGGAAT38400.066.45845
AGGGCTA14700.066.1811455
ATAGGGA32350.066.102573
Produced by FastQC (version 0.11.5)