FastQCFastQC Report
Mon 27 Feb 2023
SRR3128022.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128022.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262993
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT776123.429617325373963No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT68310.30185687715339815No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT63090.27879008021677487No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT54080.23897555140471047No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39650.17521044033278052No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG35280.15589973101993687No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTC31310.13835659235357775No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC29270.12934198205650657Illumina Single End Adapter 1 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC28210.12465791984332254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT228550.089.912861
ATAGGGC13550.076.6529543
AGGGATG32500.072.448655
AGGGAAT23250.070.5469745
GAATAGG12700.068.933211
TAGGGAT14700.068.738024
TAGGGCA9900.068.360184
AGTAGGG34600.068.22652
GGTAAGG12450.068.049111
AGAGGGC34050.067.356363
ATAGGGA18700.067.354893
CGTTTCT11400.067.297991
GTAGGGA16400.067.057593
AGATAGG12850.066.6634141
ATAGGGT9350.066.3495943
GATAGGG31100.065.774022
AGTAAGG15400.065.405211
ATGCGGG10500.065.386642
GTAGGGT8650.065.199023
TAGGGTA7000.065.125284