FastQCFastQC Report
Mon 27 Feb 2023
SRR3128021.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128021.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132829
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT712763.3418525348258115No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT72200.33851752765927323No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT67400.31601220726087276No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT57200.26818840141427186No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG38230.17924549975642678No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34710.16274159813093314No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTC33680.15791233146210973No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC31520.14778493728282951Illumina Single End Adapter 1 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC28950.13573521365285263No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT210100.089.5564651
ATAGGGC13050.073.1028753
AGGGATG28900.073.0142445
AGTAGGG30400.071.728752
GAATAGG13200.068.0477751
ATAGGGA16050.067.930043
CGTTTCT11450.067.769291
TAGGGCA10500.067.5828554
GAGGGAT23300.067.16414
GATAGGG28300.066.4236152
AAGAGGG65900.065.821112
AGTAAGG11650.065.798521
AATAGGG29400.065.696682
ACGGGAT5750.065.3839344
AGGGAAT23100.065.305865
AGGGACT24350.065.234425
TAGGGTA6200.065.1861954
TATAGGG15650.065.161992
AGAGGGC31500.064.8974463
GGTAAGG11200.064.663191