FastQCFastQC Report
Mon 27 Feb 2023
SRR3128020.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128020.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632784
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT992543.7699256756346133No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT71420.27127177922685647No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT66630.2530781104716528No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT55280.20996785152143133No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT44590.1693644446335134No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG38180.14501759354356453No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTC33330.1265960291463333No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC29790.11315018626670474Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT279300.090.106671
ATAGGGC17400.071.581033
AGGGATG40700.070.441495
GAATAGG15250.070.3428961
ATAGAGG19950.070.279491
ATAGGGA23200.069.892813
TATAGGG20050.069.387092
AGGGAAT30850.068.5567865
TAGAGGG50050.068.458092
GGTAAGG13550.068.4041061
GAGGGAT30250.067.742694
TAAGGGA21750.067.637333
ACGGGAT6450.066.3106164
AGTAGGG37900.066.222172
CGTTTCT11050.065.997081
AGGGACT32500.065.7992865
AGAGGGC39900.065.3764653
AGATAGG13850.064.8841861
TAGGGAT17900.064.855274
AAGAGGG87650.064.561852