FastQCFastQC Report
Mon 27 Feb 2023
SRR3128019.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128019.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086168
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780903.743226815865261No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCT56450.27059182194339093No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT56090.26886616993454027No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT49040.23507215142788118No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31960.15319955056352125No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG28690.13752487814979428No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTC24790.11883031472057859No Hit
CGTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTC21750.10425814220139511No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT230100.090.5612641
AGGGATG28300.073.5691765
ATAGGGC12150.072.334243
TAGGGCA8300.071.346234
TAGAGGG36700.069.318522
AGTAGGG30000.069.2817842
ATAGGGA14400.068.8653
TAGGGTA6050.068.360614
GAATAGG12050.068.344331
GAGGGAT19650.068.164924
AGAGGGC31050.068.112923
AGGGACT24650.067.684625
CGTAGGG7450.067.537592
ACGGGTA2100.067.139884
GTAGGGA15650.066.968283
AGATAGG10200.066.898951
ATAGAGG13400.066.726791
GTTTTTT315850.066.6983642
CGTTTCT10100.066.629441
ATAGCGG4100.066.572621