FastQCFastQC Report
Mon 27 Feb 2023
SRR3128018.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128018.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1974644
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT719183.6420742169221385No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT62730.31767751554204204No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCT60160.3046625113184959No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT55100.27903763918964636No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG31430.1591679310295932No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28720.14544393824912236No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTC27740.14048101835064955No Hit
CGTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTC24080.12194603179104689No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC20950.106095073339802No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT202850.090.238121
TAGGGCA7900.075.546664
ATAGGGC12450.074.3593443
AGGGATG28700.072.537155
ATAGAGG12350.071.9574051
GTAGGGC8950.071.9343263
ACGGGTA2200.070.490394
AGATAGG11000.068.392421
GGTAAGG11150.068.315741
TAGGGAT10900.068.1191564
AGTAAGG12200.067.8318251
AGGGAAT20200.067.698695
TAGCGGG11100.067.315152
AGTAGGG27950.067.25382
AGAGGGC29200.067.110713
TAGAGGG35000.066.8651662
TATAGGG15200.066.780372
GTTTTTT275800.066.690692
GAGGGAT20450.066.641284
TAAGGGA17700.065.5786363