FastQCFastQC Report
Mon 27 Feb 2023
SRR3128017.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128017.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464461
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1003274.07095101119474No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCT63210.25648610385800386No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT61020.24759977942438527No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT55390.224755027569923No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37170.15082405442812852No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG32480.13179352402005956No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTC29250.11868720990106964No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT284150.090.432871
ATAGGGC16150.073.922693
TAGGGCA11750.073.603174
AGTAGGG36650.071.561072
GTAGGGT9600.071.4822543
AGGGATG37500.071.31345
ATAGGGA19900.069.440183
AGGGAAT25200.068.6337055
ATAGCGG4350.068.123041
CGTTTCT11950.067.308661
TAGGGTA8400.067.145764
GTTTTTT384700.067.112592
AGTAAGG14400.066.962881
CGTAGGG8150.066.898592
GAATAGG14250.066.677491
TAGGGAT15600.066.5862054
ATAGAGG17250.066.533971
TAGAGGG43550.066.482782
TAGGGTC6450.066.312944
TAGCGGG13400.066.293912
Produced by FastQC (version 0.11.5)