FastQCFastQC Report
Mon 27 Feb 2023
SRR3128015.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128015.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2199104
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770233.5024719158348128No Hit
CGTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTT86500.3933420156572859No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCT82110.3733793399493612No Hit
CGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT69830.3175384156456448No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG50350.22895688425831612No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTC38770.1762990745321731No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34900.15870099822473152No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC34420.15651829108582405No Hit
CGTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTC33620.15288044585431157No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG22900.10413331975204446No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT217500.090.331911
CGTAGGG6100.075.500822
ATAGGGC13650.075.399223
TAGGGCA8600.073.22544
GAATAGG11750.072.839261
AGTAGGG31450.072.4730452
ATAGGGA15000.071.433023
GTAGGGT8650.070.085643
ACGGGTA2750.070.065884
AGGGATG29000.069.84495
AGATAGG11400.068.888011
ATAGAGG14400.068.252061
AGGGAAT23950.067.304485
AGTAAGG12300.067.28831
GATAGGG27000.066.8379062
CGTTTCT12900.066.7103651
GCGTAGG3750.066.462491
TATAGGG16450.066.279242
AGAGGGC33200.066.104993
GTTTTTT298900.066.067152