FastQCFastQC Report
Mon 27 Feb 2023
SRR3128013.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128013.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2163042
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169860.7852829487360855No Hit
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG39900.18446243762257045No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34290.15852674150571278No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG33230.15362623564406053No Hit
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC31330.14484231004298576Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT53650.081.127121
ATAGGGC13150.075.7672963
ATAGCGG4650.072.857381
ACGGGTA2850.072.557034
CGGGTAT2600.072.303345
CGTAGGG6450.071.4395752
TAGGGCA11100.071.1308444
AGTAGGG32050.071.005152
TAGAGGG34950.069.014882
TAAGGGA21150.067.99593
TAGCGGG9650.067.239482
TACGAGG4350.067.065081
GTAGGGA19350.066.3060763
TAGGGCG4900.066.179694
ATAGAGG13000.065.513271
GAATAGG12950.065.402861
CGAAGGG19550.065.177112
AAGGGAC19600.064.980784
GTAGGGT7600.064.93033
AGGGAAT27600.064.7062455
Produced by FastQC (version 0.11.5)