FastQCFastQC Report
Mon 27 Feb 2023
SRR3128012.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128012.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999977
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151200.7560086940999822No Hit
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG45260.2263026024799285No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG37930.18965218100008152No Hit
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC33660.1683019354722579Illumina Single End Adapter 2 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30680.1534017641202874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT46050.082.022761
CGTAGGG6850.075.467432
ATAGGGC13200.073.341663
ATAGCGG3150.073.1701661
TAGTAGG9100.072.36611
ACGGGTA1900.071.730164
AGTAGGG31800.070.050082
TACGGGT1900.069.256713
TAGGGCA10850.067.569754
ATAACGG1400.067.197091
GTAAGGG29550.066.7958152
AGTAAGG12900.066.363641
TAAGGGA19850.065.817553
AGGGAAT25850.065.812065
GAATAGG11950.065.341441
ATAGGGA18800.064.993953
GTAGGGT6800.064.964543
AGGGATG21650.064.903895
AGAGGGC25900.064.7777563
CGAAGGG17300.064.652942
Produced by FastQC (version 0.11.5)