FastQCFastQC Report
Mon 27 Feb 2023
SRR3128011.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128011.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2490554
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229800.9226862778321611No Hit
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG45880.18421604189268734No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG40670.16329700139005215No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39050.1567924244967184No Hit
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC36660.1471961659935902Illumina Single End Adapter 2 (95% over 21bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26910.10804824950593321No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69150.081.083831
ATAGCGG6050.074.639041
ATAGGGC13350.072.523463
CGTAGGG8050.071.228952
AGTAGGG37750.069.7212
TAGAGGG40700.068.0163652
ATAGGGA23650.067.567893
AGTAAGG17750.067.310921
CGAAGGG20650.067.142042
GTAGGGT9500.066.7886663
TAGTAGG9950.066.6570741
AGAGGGC33450.066.1785663
TAAGGGA25500.066.1678163
TGTAGGG18150.065.773312
TAGGGCA12650.065.390514
AAGAGGG79900.065.293322
AGGGAAT31150.065.031015
AAGGGAC23000.064.777474
ACGGGAT5750.064.573134
GAATAGG15550.064.431681
Produced by FastQC (version 0.11.5)