FastQCFastQC Report
Mon 27 Feb 2023
SRR3128010.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128010.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3040396
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267170.8787342175164023No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG48440.15932135156078353No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40540.13333789414273667No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG40370.13277875645146225No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC36650.12054350814828067Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85050.079.550871
CGTAGGG7950.078.065632
GGTAAGG16650.074.8845141
ATAGGGC18400.074.5853963
TAGGGCA17100.072.8347244
TAGGGTA12500.071.43854
AGTAGGG53650.071.24822
GTAGGGT11800.069.303633
TATAGGG23950.069.298252
AGTACGG3950.069.08621
AGGGATG45400.069.049215
GAATAGG16350.068.488811
TAGAGGG52000.068.44562
GTAGGGC17850.068.194653
GTACGGG7250.068.093292
ATAGAGG19400.067.907341
TAAGGGA25750.067.3500443
AGGGTAC15750.066.843045
AGGGAAT32450.066.769015
AAGGGAT54600.065.936354
Produced by FastQC (version 0.11.5)